ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (11): 2063-2068.doi: 10.11843/j.issn.0366-6964.2015.11.020

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Identification of High Immunogenic Components of Toxoplasma gondii Excreted-secreted Antigens

YE Qiang1,WANG Meng1,ZHANG Nian-zhang1,YIN Hong1,2,ZHANG De-lin1,2*   

  1. (1.State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;2.Jiangsu Co-innovation Center for Prevention and Central of Important Animal Infectious Disease,Yangzhou 225009,China)
  • Received:2015-02-12 Online:2015-11-23 Published:2015-11-23

Abstract:

The objective of this study was to screen the main immunogenic antigen of Toxoplasma gondii excreted-secreted antigen(ESA).2-DE combined with Western blotting were used to analysis the components of T.gondii ESA.Eighteen positive protein points were detected in this study.Among them,6 protein spots were selected to identify by matrix-assisted laser desorption/lionization time of flight mass spectrometry(MALDI-TOF/TOF),belonging to microneme protein 1,microneme protein 4,cyst matrix protein and 14-3-3 protein.This study provides a new reference for looking for new candidate molecules for prevention and diagnosis of toxoplasmosis.

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